Translational_Unit

Part:BBa_K5179212:Design

Designed by: Rachel McCarley   Group: iGEM24_William-and-Mary   (2024-09-30)


HerbertWM Gp3 Mutant


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 747
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 735
    Illegal XhoI site found at 186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 747
    Illegal NgoMIV site found at 966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 389
    Illegal BsaI.rc site found at 189


Design Notes

None


Source

Made from concatenated protein domains of phage L5 and HerbertWM. The DNA used to assemble this part was obtained from IDT (Integrated DNA Technologies).

References